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Preparation of trastuzumab and ADC aggregates Trastuzumab and ADC aggregates were prepared by diluting monoclonal antibody in mobile phase to a final concentration of 2 mgmL. pH stress was carried out as described elsewhere with slight modification 3. Briefly, 1 M HCl was slowly added dropwise to the sample solutions to change the pH from 6
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Protein aggregation can be reversible or irreversible and the aggregates formed can be solubleinsoluble, covalentnoncovalent and nativenonnative Figure 13.1 2227. Nonnative protein aggregation is the typical mechanism encountered in the biopharmaceutical industry and it will be termed protein aggregation in this chapter 23 .
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The alkalisilica reaction ASR, more commonly known as 34concrete cancer34, is a swelling reaction that occurs over time in concrete between the highly alkaline cement paste and the reactive noncrystalline silica found in many common aggregates, given sufficient moisture.. This reaction causes the expansion of the altered aggregate by the formation of a soluble and viscous gel of sodium
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